| Chemical Properties |
|
| [Appearance]
White to pale yellow |
| [Melting point ]
95-98 °C(lit.) |
| [Boiling point ]
215.33°C (rough estimate) |
| [density ]
1.4769 (rough estimate) |
| [vapor pressure ]
0Pa at 25℃ |
| [refractive index ]
1.4080 (estimate) |
| [storage temp. ]
2-8°C |
| [solubility ]
DMSO (Soluble), Methanol (Slightly) |
| [form ]
Liquid or Solid |
| [pka]
7.81±0.20(Predicted) |
| [color ]
Clear yellow-brown to brown |
| [PH]
5-7 (50g/l, H2O, 20℃) |
| [Stability:]
Stable. Incompatible with strong oxidizing agents, acid chlorides, acid anhydrides, strong bases. Protect from moisture. |
| [Water Solubility ]
SOLUBLE |
| [Sensitive ]
Hygroscopic |
| [Usage]
Additive used in the carbodiimide method for improved amidations and peptide couplings. |
| [Detection Methods]
T,NMR |
| [BRN ]
113913 |
| [InChIKey]
NQTADLQHYWFPDB-UHFFFAOYSA-N |
| [LogP]
-2.01 |
| [Uses]
N-hydroxysuccinimide (NHS) is often used to assist carbodiimide-mediated peptide coupling by forming an active ester intermediate via condensation of the surface carboxyl group and NHS. The NHS-reactive ester intermediate is susceptible to nucleophilic attack by primary amines and results in the formation of stable amide bonds between the biomaterial surface and the N-terminus of the peptide. |
